Identification of asialo GM1 as a binding structure for Escherichia coli colonization factor antigens

HS Orø, AB Kolstø, C Wennerås… - FEMS microbiology …, 1990 - academic.oup.com
HS Orø, AB Kolstø, C Wennerås, AM Svennerholm
FEMS microbiology letters, 1990academic.oup.com
We have examined the binding of colonization factor antigens,(CFAs) of enterotoxigenic
Escherichia coli to gangliosides and asialo gangliosides by using immuno-thin layer
chromatography. CFA/II and its subcomponents (CS1, CS2 and CS3) as well as the
subcomponent CS4 of the CFA/IV complex bound to asialo ganglioside GM1.
Summary
We have examined the binding of colonization factor antigens, (CFAs) of enterotoxigenic Escherichia coli to gangliosides and asialo gangliosides by using immuno-thin layer chromatography. CFA/II and its subcomponents (CS1, CS2 and CS3) as well as the subcomponent CS4 of the CFA/IV complex bound to asialo ganglioside GM1.
Oxford University Press