In vitro induction of specific cytotoxic T lymphocytes using recombinant single-chain MHC class I/peptide complexes
YC Lone, I Motta, E Mottez, Y Guilloux… - Journal of …, 1998 - journals.lww.com
We have previously described the production and purification of a murine single-chain,
soluble recombinant major histocompatibility complex (MHC) class I molecule (SC-Kd). A
similar strategy was devised to produce a recombinant HLA-A2. 1 (SC-A2) molecule. The
latter was composed of the first three domains of the HLA-A2. 1 heavy chain connected to
human [beta] 2-microglobulin through a spacer of 15 amino acids. Immunoaffinity-purified
SC-A2 molecules were correctly folded and biologically functional. They specifically bound …
soluble recombinant major histocompatibility complex (MHC) class I molecule (SC-Kd). A
similar strategy was devised to produce a recombinant HLA-A2. 1 (SC-A2) molecule. The
latter was composed of the first three domains of the HLA-A2. 1 heavy chain connected to
human [beta] 2-microglobulin through a spacer of 15 amino acids. Immunoaffinity-purified
SC-A2 molecules were correctly folded and biologically functional. They specifically bound …