Single-cell analysis of cytokine production shows different immune profiles in multiple sclerosis patients with active or quiescent disease
M Clerici, M Saresella, D Trabattoni, L Speciale… - Journal of …, 2001 - Elsevier
Peripheral blood mononuclear cells of multiple sclerosis (MS) patients were stimulated with
myelin basic protein (MBP) together with anti-CD28 monoclonal antibody and
staphylococcal enterotoxin B to optimize cytokine production by antigen-specific cells. Type
1 (IL-2, IL-12, IFNγ) and pro-inflammatory (TNFα, IL-1β, IL-6) cytokines were augmented in
CD4+, CD8+, and CD14+ cells of acute MS patients and of patients undergoing disease
reactivation. These cytokines were reduced in IFNβ-treated and in stable MS patients; type 2 …
myelin basic protein (MBP) together with anti-CD28 monoclonal antibody and
staphylococcal enterotoxin B to optimize cytokine production by antigen-specific cells. Type
1 (IL-2, IL-12, IFNγ) and pro-inflammatory (TNFα, IL-1β, IL-6) cytokines were augmented in
CD4+, CD8+, and CD14+ cells of acute MS patients and of patients undergoing disease
reactivation. These cytokines were reduced in IFNβ-treated and in stable MS patients; type 2 …
