Protein kinase C inhibits ROMK1 channel activity via a phosphatidylinositol 4, 5-bisphosphate-dependent mechanism
WZ Zeng, XJ Li, DW Hilgemann, CL Huang - Journal of Biological …, 2003 - ASBMB
The activity of apical K+ channels in cortical collecting duct (CCD) is stimulated and inhibited
by protein kinase A (PKA) and C (PKC), respectively. Direct interaction between
phosphatidylinositol 4, 5-bisphosphate (PIP 2) and the cloned CCD K+ channel, ROMK1, is
critical for channel opening. We have found previously that phosphorylation of ROMK1 by
PKA increases affinity of the channel for PIP 2 and mutation of PKA sites reduces the affinity
of ROMK1 for PIP 2. In this study we investigate the molecular mechanism for PKC …
by protein kinase A (PKA) and C (PKC), respectively. Direct interaction between
phosphatidylinositol 4, 5-bisphosphate (PIP 2) and the cloned CCD K+ channel, ROMK1, is
critical for channel opening. We have found previously that phosphorylation of ROMK1 by
PKA increases affinity of the channel for PIP 2 and mutation of PKA sites reduces the affinity
of ROMK1 for PIP 2. In this study we investigate the molecular mechanism for PKC …
