Generation of Mammalian Host-Adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats
MJ Caimano - Borrelia burgdorferi: Methods and Protocols, 2018 - Springer
Borrelia burgdorferi: Methods and Protocols, 2018•Springer
The transmission, survival, and virulence of Borrelia burgdorferi depend upon the
spirochete's ability to modulate its transcriptome as it cycles between its arthropod vector
and reservoir host. This complex adaptive process is collectively referred to as “host-
adaptation.” The paucibacillary nature of borrelial infections precludes the detailed analysis
of host adaptation within infected mammalian tissues. To circumvent this limitation, we (J
Clin Invest 101: 2240–2250, 1998) developed a model system whereby spirochetes are …
spirochete's ability to modulate its transcriptome as it cycles between its arthropod vector
and reservoir host. This complex adaptive process is collectively referred to as “host-
adaptation.” The paucibacillary nature of borrelial infections precludes the detailed analysis
of host adaptation within infected mammalian tissues. To circumvent this limitation, we (J
Clin Invest 101: 2240–2250, 1998) developed a model system whereby spirochetes are …
Abstract
The transmission, survival, and virulence of Borrelia burgdorferi depend upon the spirochete’s ability to modulate its transcriptome as it cycles between its arthropod vector and reservoir host. This complex adaptive process is collectively referred to as “host-adaptation.” The paucibacillary nature of borrelial infections precludes the detailed analysis of host adaptation within infected mammalian tissues. To circumvent this limitation, we (J Clin Invest 101:2240–2250, 1998) developed a model system whereby spirochetes are cultivated within dialysis membrane chambers (DMCs) surgically implanted within the peritoneal cavity of a rat. Spirochetes within DMCs are exposed to many, if not all, of the environmental signals and physiological cues required for mammalian host adaptation but are protected from clearance by the host’s immune system.
Springer