[HTML][HTML] Expression and one-step purification of active LPL contemplated by biophysical considerations
AML Winther, KK Kristensen, A Kumari… - Journal of Lipid Research, 2021 - ASBMB
LPL is essential for intravascular lipid metabolism and is of high medical relevance. Since
LPL is notoriously unstable, there is an unmet need for a robust expression system
producing high quantities of active and pure recombinant human LPL (hLPL). We showed
previously that bovine LPL purified from milk is unstable at body temperature (T m is 34.8°
C), but in the presence of the endothelial transporter glycosylphosphatidylinositol-anchored
high density lipoprotein-binding protein 1 (GPIHBP1), LPL is stabile (T m increases to 57.6° …
LPL is notoriously unstable, there is an unmet need for a robust expression system
producing high quantities of active and pure recombinant human LPL (hLPL). We showed
previously that bovine LPL purified from milk is unstable at body temperature (T m is 34.8°
C), but in the presence of the endothelial transporter glycosylphosphatidylinositol-anchored
high density lipoprotein-binding protein 1 (GPIHBP1), LPL is stabile (T m increases to 57.6° …